P-217: Expression Analysis of The Histone Variant H2A.Z in Endometrium Tissue during The Menstrual Cycle

Background: The human endometrium undergoes cyclical changes including proliferation, differentiation, tissue breakdown and shedding (menstruation) throughout a woman’s reproductive life. The postovulatory rise in ovarian progestrone induces profound remodeling in chromatin structure of cells, and consequently differentiation of estradiol-primed endometrium. This change is crucial for embryo implantation and maintenance of pregnancy. H2A.Z is a histone variant that plays role in cellular proliferation. Some studies has recently demonstrated the importance of the presence of H2A.Z in chromatin to cell-cycle progression via the p53/p21 pathway. H2A.Z overexpression has been associated functionally with the proliferation state of cells. It is an important regulator of gene expression, and its deregulation may lead to the increased proliferation of mammalian cells. in the current work, expression analysis H2A.Z of histone variant has been evaluated in women in prolifrative and secretory phases. Materials and Methods: Local ethical approval was gained for this study and informed consent was given by patients. All the women taking part in the investigation had regular cycles, showed no evidence of any pathological uterine disorder and had not used oral contraception or an intrauterine device in the previous 3 months. Cellular RNA was extracted from tissue samples and cDNA synthesis was performed on them. The expression level of H2A.Z was evaluated by the use of qRT-PCR techniqe. Results: The results showed that the mRNA level of H2A.Z was significantly higher in prolifrative phase compared to secretory phase. Conclusion: This finding implies for the first time that the histone variant H2A.Z can be considered as an epigenetic factor in endometrium reconstruction during the menstrual cycle.